Submitted by Lauren French (download pdf)

Clean and sterilize the hood using 70% ethanol.

In water bath (37 °C), warm for 1 hour Complete Media and other necessary media

 

To Feed Cells:

Using pre-sterilized Louis Pipette attached to vacuum, remove media from plate/flask

Quickly add Complete Media to plate using sterile Falcon Pipet with Pipet-Aid, only feeding 1-3plates of same cells at a time to avoid drying cells.

On the plate cover, write the date fed and initial

Return plate to incubator

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